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miltenyi biotec cd3 fitc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec miltenyi biotec cd3 fitc
    Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, <t>CD3</t> + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.
    Miltenyi Biotec Cd3 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd3+fitc/CD3+Antibody%2C+anti-human%2C+REAfinity/pmc13255789-204-15-15
    Average 95 stars, based on 366 article reviews
    miltenyi biotec cd3 fitc - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production"

    Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production

    Journal: Biology

    doi: 10.3390/biology15110848

    Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, CD3 + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.
    Figure Legend Snippet: Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, CD3 + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.

    Techniques Used: Generated, Plasmid Preparation, Produced, Flow Cytometry, Selection, Transduction

    Related Articles

    Staining:

    Article Title: Isoproterenol infusion enhances composition and function of G-CSF mobilized allogeneic peripheral blood hematopoietic cell grafts
    Article Snippet: .. For flow cytometric analysis, 25μL of whole blood samples were stained with directly conjugated antibodies, including CD8-VioBlue, CD14-VioGreen, CD3-FITC, CD4-PE, CD20-PerCP, CD45mouse-PE-Vio770, CD45human-APC, and CD56-APC-Vio770 (all from Miltenyi Biotec). ..

    Article Title: Relations of Residential Greenness with Immunity and Inflammation.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Tumor microenvironment remodeling by STING agonism sensitizes endothelial cells to cytotoxic anti-PD-L1/L2 antibody.
    Article Snippet: Fc receptors were blocked using anti-mouse CD16/CD32 antibody (ThermoFisher Scientific). .. Surface staining was performed at 4 °C for 30 minutes using various combinations of the following fluorophore-conjugated antibodies: CD45-VioGreen, CD11b-FITC, CD11c-APC, F4/80-PE-Vio770, Ly6C-APC/Vio770, Ly6G-VioBlue, CD3-FITC, CD4-APC/Vio770, CD8-VioBlue, PD-1-APC (from Miltenyi Biotec) and CD44-PE, CD206-PE, PD-L1-PE, PD-L1-APC, PD-L1-PeFire700, PD-L2-PE, PD-L2 BUV563 or PD-L2-PECF594 and CD86 (GL1, Brilliant Violet, BV, 711) were from BioLegend. .. 14 AR TIC LE IN PR ES S Page | CD206 (BV 650), PD-L1 (CD274, PE-Fire 700), CD14 (BV 421), CD8a (BV 510), FoxP3 (PE) were from BioLegend; CD45 (AlexaFluor 647), F4/80 (Brilliant Ultraviolet 496), PD-L2 (CD273, Brilliant Ultraviolet 563), CD11c (Brilliant Violet 786), CD86 (Brilliant Violet 711), CD3 (Brilliant Ultraviolet 395), PD-1 (RealBlue 705), were from BD Biosciences; Fixable Live/Dead (Live/Dead Fix Near IR 775), Ly-6G (Brilliant Ultraviolet 737), CD45 (Brilliant Violet 786), CD4 (Brilliant Ultraviolet 737), and CD44 (Brilliant Ultraviolet 615) (from Thermo Fisher); CD11b (StarBright UV445) and CD19 (StarBright Blue 615) were from Bio-Rad; Ly-6C (Vio Bright 515) and CD25 (Vio Bright 667) were from Miltenyi Biotec.

    Article Title: TIGIT Blockade Potentiates the Anti-Leukemic Activity of Exercise-Mobilized Donor Lymphocytes and Expanded γδ T-Cells.
    Article Snippet: .. 25 μL of whole blood samples were stained and analyzed by flow cytometry using the following directly conjugated antibodies: CD3-FITC, CD45mouse-PE-Vio770, CD45human-APC, TCR-γδ PerCP-Vio700 (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: TIGIT Blockade Potentiates the Anti-Leukemic Activity of Exercise-Mobilized Donor Lymphocytes and Expanded γδ T-Cells
    Article Snippet: .. 25 μL of whole blood samples were stained and analyzed by flow cytometry using the following directly conjugated antibodies: CD3-FITC, CD45mouse-PE-Vio770, CD45human-APC, TCR-γδ PerCP-Vio700 (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Flow Cytometry:

    Article Title: Ibrutinib increases miR-181a/b in leukemic cells from patients with chronic lymphocytic leukemia
    Article Snippet: .. PBMC was characterized by flow cytometry using the following antibodies: CD19-PECy5 (555414, BD), CD19-BV421 (562440, BD), CD19-APC-H7 (388814, BD), CD5-APC (555353, BD), CD3- FITC (130-080-401, Miltenyi Biotec), CD3-PECy7 (557851, BD), CD8-APC (345775, BD), Granzyme BB-BV510 (563388, BD). .. Data were acquired with a FACSCanto II, FACSVerse cell analyzer (BD Biosciences) and analyzed with FlowJo v10.0.7 and/or Kaluza analysis software (Beckman).

    Article Title: TIGIT Blockade Potentiates the Anti-Leukemic Activity of Exercise-Mobilized Donor Lymphocytes and Expanded γδ T-Cells.
    Article Snippet: .. 25 μL of whole blood samples were stained and analyzed by flow cytometry using the following directly conjugated antibodies: CD3-FITC, CD45mouse-PE-Vio770, CD45human-APC, TCR-γδ PerCP-Vio700 (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: TIGIT Blockade Potentiates the Anti-Leukemic Activity of Exercise-Mobilized Donor Lymphocytes and Expanded γδ T-Cells
    Article Snippet: .. 25 μL of whole blood samples were stained and analyzed by flow cytometry using the following directly conjugated antibodies: CD3-FITC, CD45mouse-PE-Vio770, CD45human-APC, TCR-γδ PerCP-Vio700 (Miltenyi Biotec, Bergisch Gladbach, Germany). ..



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    Image Search Results


    Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, CD3 + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.

    Journal: Biology

    Article Title: Development of Replicon Cell Pools Bearing a Flavivirus RNA Replicon as a Source of HIV-1 Gag-Pol for Lentiviral Vector Production

    doi: 10.3390/biology15110848

    Figure Lengend Snippet: Characterization of a CAR-T cell product generated using LV/CAR vector produced from RCP cells. ( a ) Representative flow cytometry plots showing the gating strategy used for counting CAR + cells (debris exclusion, CD45 + leukocytes, viable cells, CD3 + T cells, and CAR + subset within CD3 + cells). ( b ) Summary of cell product composition after T-cell selection and transduction, including cell subsets, markers, event counts, and relative frequencies.

    Article Snippet: The CAR-T cell product was analyzed by flow cytometry using the following antibody panel from Miltenyi Biotec: CD3-FITC (130-113-138), CD4-VioGreen (130-113-221), CD8-APC-Vio770 (130-113-155), CD14-APC (130-113-143), CD45-VioBlue (130-113-122), biotinylated CD19 CAR Detection Reagent (130-129-550), anti-biotin- phycoerythrin (PE)-conjugated antibody (130-113-291).

    Techniques: Generated, Plasmid Preparation, Produced, Flow Cytometry, Selection, Transduction

    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Journal: Molecular Therapy Oncology

    Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

    doi: 10.1016/j.omton.2026.201185

    Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

    Techniques: Flow Cytometry

    Knockdown of LDHA/B inhibits HCC growth by activating CD8 + T cell-mediated antitumor immunity (A) WB analysis of LDHA, LDHB, and β-actin (loading control) expression in control and LDHA/B DKO Hepa 1–6 cells. (B) Representative images of tumors dissected from tumor-bearing mice ( n = 6). Tumor weights (C), tumor growth curves (D), Kaplan-Meier survival curve ( n = 6) (E), and lactate contents in tumor tissues (F) of the control and LDHA/B DKO groups ( n = 6). (G) IHC staining of H3K18la and Pan Kla in tumor tissues (scale bars, 100 μm, n = 6). (H–M) FC analysis of tumor-infiltrating immune cells: TILs were isolated from control and LDHA/B-DKO tumors. FC plots (left) and quantitative statistics (right) show: the proportion of CD3 + CD8 + CTLs (H), the proportion of CD44hiCD62Llo effector memory CTLs (I), and the proportions of intratumoral GzMB + (J), and IFN-γ + (K) CTLs with representative scatterplots ( n = 6). Cell aggregates and debris were first excluded, followed by gating on the target cell populations and markers of interest. Representative FC plots and quantitative data are presented. (L and M) Tumor size and growth curves of mice treated with anti-CD8 antibody to deplete CD8 + T cells or isotype control (IgG) ( n = 6). (N) Mouse body weights at the end of the experiment ( n = 6). Data are presented as mean ± SD. (C–F, and H–M) were analyzed using Student’s t test, and (N) was analyzed by two-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns, not significant. All experiments were repeated three times. β-actin served as the loading control for WB experiments.

    Journal: iScience

    Article Title: Targeting KIF20A blocks lactylation modification to suppress immune escape in hepatocellular carcinoma

    doi: 10.1016/j.isci.2026.115372

    Figure Lengend Snippet: Knockdown of LDHA/B inhibits HCC growth by activating CD8 + T cell-mediated antitumor immunity (A) WB analysis of LDHA, LDHB, and β-actin (loading control) expression in control and LDHA/B DKO Hepa 1–6 cells. (B) Representative images of tumors dissected from tumor-bearing mice ( n = 6). Tumor weights (C), tumor growth curves (D), Kaplan-Meier survival curve ( n = 6) (E), and lactate contents in tumor tissues (F) of the control and LDHA/B DKO groups ( n = 6). (G) IHC staining of H3K18la and Pan Kla in tumor tissues (scale bars, 100 μm, n = 6). (H–M) FC analysis of tumor-infiltrating immune cells: TILs were isolated from control and LDHA/B-DKO tumors. FC plots (left) and quantitative statistics (right) show: the proportion of CD3 + CD8 + CTLs (H), the proportion of CD44hiCD62Llo effector memory CTLs (I), and the proportions of intratumoral GzMB + (J), and IFN-γ + (K) CTLs with representative scatterplots ( n = 6). Cell aggregates and debris were first excluded, followed by gating on the target cell populations and markers of interest. Representative FC plots and quantitative data are presented. (L and M) Tumor size and growth curves of mice treated with anti-CD8 antibody to deplete CD8 + T cells or isotype control (IgG) ( n = 6). (N) Mouse body weights at the end of the experiment ( n = 6). Data are presented as mean ± SD. (C–F, and H–M) were analyzed using Student’s t test, and (N) was analyzed by two-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001; ns, not significant. All experiments were repeated three times. β-actin served as the loading control for WB experiments.

    Article Snippet: FITC Anti-Mouse CD3 Antibody , Elabscience , Cat# E-AB-F1013C; RRID: AB_3065041.

    Techniques: Knockdown, Control, Expressing, Immunohistochemistry, Isolation